Stress affects many aspects of physical and mental health, yet physiological changes associated with stress can be difficult to monitor objectively over time. Serotonin is an important signaling molecule involved in mood regulation and stress-related physiology. It is also present in tears, making tear fluid an attractive, minimally invasive source of biochemical information. The study was designed to explore whether a soft ocular bioelectronic platform could sensitively detect serotonin in this small and dynamic biofluid.